Abstract
Abstract The complete A+T-rich region of mitochondrial DNA has been cloned and sequenced from Antheraea pernyi (Lepidoptera: Saturniidae). Nine haplotypes were obtained from 16 inbred strains of the domesticated type with the range from 550 to 554 bp in size, exhibiting a low degree of sequence divergence from 0.18 to 0.91%. Two haplotypes were observed from three individuals of the wild type with 516 or 517 bp, respectively, showing a length polymorphism and a relatively high degree of sequence divergence compared with those of the domesticated type. Phylogenetic analysis showed the presence of two clearly differentiated mitochondrial clades: the domesticated type and the wild type. The length polymorphism between the two types was caused by the presence of a variable number of tandemly repeated units. Several conserved structural elements previously described in other insects were also present in the A. pernyi A+T-rich region. Moreover, two tRNA-like structures were observed in this region.
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