Abstract
Due to the multiplicity of useful applications of metal oxide nanoparticles (ONPs) in medicine are growing exponentially, in this study, Fe3O4 (iron oxide) nanoparticles (IONPs) were biosynthesized using Rosemary to evaluate the leishmanicidal efficiency of green synthesized IONPs. This is the first report of the leishmanicidal efficiency of green synthesized IONPs against Leishmania major. The resulting biosynthesized IONPs were characterized by ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The leishmanicidal activity of IONPS was studied via 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed the fabrication of the spherical shape of monodisperse IONPs with a size 4 ± 2 nm. The UV-visible spectrophotometer absorption peak was at 334 nm. The leishmanicidal activity of biogenic iron oxide nanoparticles against Leishmania major (promastigote) was also studied. The IC50 of IONPs was 350 µg/mL. In this report, IONPs were synthesized via a green method. IONPs are mainly spherical and homogeneous, with an average size of about 4 nm, and were synthesized here using an eco-friendly, simple, and inexpensive method.
Highlights
Misuse of antibiotics may lead to the spread of resistant microbial strains
In this study, we aimed to evaluate the leishmanicidal efficiency of biosynthesized iron oxide nanoparticles (IONPs)
Increase in all three parameters led to an increase in the intensity peak of UV-visible analysis that shows the better effect of increasing these parameters on the synthesis process
Summary
Misuse of antibiotics may lead to the spread of resistant microbial strains. The increase in resistant microbial strains is becoming a serious and complex problem in medicine; it is necessary to explore new antimicrobial substances and replace them with common antibiotics [1]. Nanoparticles have attracted scientists’ interests due to their strong antimicrobial properties [2,3]. Successfully studied the bactericidal and fungicidal effect of metallic nanoparticle in real conditions. Nanobiotechnology is an emerging field of nanotechnology that utilizes nano-bio-based systems for such biological usages as the therapeutic drug delivery, immunology, biotechnology, medicine, and engineering [5,6,7,8,9]. According to the European Commission recommendations, nanoparticles are defined as manufactured materials containing particles in a free state or as an aggregate, wherein at
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