Abstract
Within the frame of molecular identification of Leishmania species, karyotyping by Pulsed Field Gradient Gel Electrophoresis (PFG) (Schwartz et al., 1983) has been applied (Spithill and Samaras, 1985; Comeau et al., 1986; Garveyand and Santi, 1986; Giannini et al., 1986; Scholler et al., 1986). In this new kind of electrophoresis, the combination of two perpendicular asymetrical electrical fields activated alternatively, induces the resolution of the nuclear genome in chromosome-sized DNA molecules, and makes it possible to obtain a karyotype of the organism being studied.
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