Leishmania sp: Comparative Study with Toxoplasma gondii and Trypanosoma cruzi in Their Ability to Initialize IL-12 and IFN-γ Synthesis

Abstract

Oliveira, M. A. P., Santiago, H. C., Lisboa, C. R., Ceravollo, I. P., Trinchieri, G., Gazzinelli, R. T, and Vieira, L. Q. 2000. Leishmania sp: Comparative study with Toxoplasma gondii and Trypanosoma cruzi in their ability to initialize IL-12 and IFN-γ synthesis. Experimental Parasitology95, 96–105. We compared in vitro and in vivo induction of IL-12 (p40) and IFN-γ by mouse cells stimulated with Toxoplasma gondii, Trypanosoma cruzi, and different species of Leishmania. Spleen cells cultured in vitro with T. cruzi or T. gondii, but not with Leishmania, produced IL-12 (p40) and IFN-γ. Accordingly, IL-12 (p40) was produced by macrophages stimulated in vitro with live T. cruzi or T. gondii or membrane glycoconjugates obtained from trypomastigotes or tachyzoites. No IL-12 production was detected when macrophages were stimulated with live parasites or glycoconjugates from Leishmania, regardless of priming with IFN-γ. In vivo, only T. cruzi and T. gondii induced the synthesis of IL-12 and IFN-γ by mouse spleen cells after intraperitoneal injection of parasites. When injected subcutaneously, live Leishmania sp. induced IL-12 (p40) and IFN-γ production by draining lymph node cells, albeit the levels were slightly lower than those induced by infection with T. gondii or T. cruzi using the same route. Together our results indicate that under different conditions, the intracellular protozoa T. gondii and T. cruzi are more potent stimulators of IL-12 and IFN-γ synthesis by host immune cells than parasites of the genus Leishmania.