Abstract
1. 1. Decarboxylation of polyamine precursors: l-omithine and l-methionine was determined along the growth curve of Leishmania infantum promastigotes in vivo, reaching maximum values on day 2 post-inoculum (mid-logarithmic phase). 2. 2. Maximum values of l-ornithine and l-methionine decarboxylation were: 1.97 ± 0.28 nmol CO 2/hr/10 7 promastigotes and 3.18 ±0.34 nmol CO 2/hr/10 7 promastigotes, respectively. 3. 3. Total (free + conjugated) polyamine content was closely related with the proliferative stage of Leishmania infantum promastigotes. 4. 4. d, l-alpha-difluoromethylomithine (DFMO) and Berenil depleted putrescine levels in a concentrationdependent manner. 5. 5. Total and free putrescine/spermidine ratio varied significantly with the proliferative stage. Minimum values were found in late logarithmic phase (day 3 post-inoculum). 6. 6. Small but detectable amounts of free spermine were detectable along the growth curve of Leishmania infantum promastigotes.
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