Abstract
Long non-coding (lncRNA) lymphoid enhancer-binding factor 1 antisense RNA 1 (LEF1-AS1) has been validated to be implicated in manifold cancers, whereas its function in glioma has not been understood thoroughly. Hence, in this study, we tested that LEF1-AS1 expression was significantly upregulated in glioma tissues and cell lines. Besides, knockdown of LEF1-AS1 repressed cell proliferation while activated apoptosis in glioma cells in vitro, and also suppressed tumor growth in vivo. RNA pull-down and luciferase reporter assays affirmed that LEF1-AS1 could bind with miR-489-3p. In addition, miR-489-3p expression was downregulated in glioma cells. Moreover, miR-489-3p depletion partly offset LEF1-AS1 knockdown-mediated function on proliferation and apoptosis. Further, HIGD1A identified as the target gene of miR-489-3p was upregulated in glioma cells. HIGD1A silence could restrict the process of glioma. In rescue assays, upregulation of HIGD1A remedied the inhibitory impacts of LEF1-AS1 silence on glioma cell growth. In summary, our studies corroborated the regulatory mechanism of LEF1-AS1/miR-489-3p/HIGD1A axis in glioma, suggesting that targeting LEF1-AS1 might be a promising method for glioma therapy in the future.
Highlights
Glioma is a common brain tumor in adults, accounting for about 60%1
The colony formation assay revealed that LEF1-AS1 silence remarkably decreased glioma cell proliferation (Fig. 1c)
In the late decades, the crucial influence of long non-coding RNAs (lncRNAs) in glioma has been well-accepted such as its modulation on metastasis, chemoresistance and growth[17,18]
Summary
Glioma is a common brain tumor in adults, accounting for about 60%1. The distinct symbols of high-grade glioma contain neurocognitive impairment, poor quality of life as well as loss of life independence ability[2]. Surgery has achieved great achievements in combination with radiotherapy and medication, the recurrence rate of glioma patients is still increasing[3,4]. A number of literatures supported that long non-coding RNAs (lncRNAs) play an important role in the occurrence and development of various cancers, including glioma. Serum lncRNA HOTAIR serves as a new biomarker for the diagnosis and prognosis of glioblastoma multiforme[5]. LncRNA NEAT1 knockdown represses migration and invasion of glioma cells through regulating SOX2 targeted by miR-1326. LEF1-AS1 was abnormally upregulated in glioma tissues and closely associated with unsatisfactory prognosis of glioma patients via public database. Present research confirmed that LEF1-AS1 was remarkably upregulated in tissues from glioma patient. The function of LEF1-AS1 in glioma has not been elucidated yet
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