Lee allergènes du pollen de Graminées. Effets de l'environnement sur l'allergénicité

Abstract

SummaryWe have studied the heterogeneity of the grass Dactylis glomerata pollen allergens by the IgE antibodies from more than 3000 allergic patient sera. We have developped in 1980 the nitrocellulose immunoprint technique to study the allergens from a pollen crude extract, separated by isoelectric focusing (IEF) in thin agarose gels. Among the most frequently recognized allergens, or major allergens, we have mainly studied Dac g I and Dac g X. These allergens were purified and characterized by their isoelectric points (6.12 and 10.4) and their relative molecular mass (32 and 12 kDa). Mouse monoclonal antibodies specific to these allergens were prepared to allow their immuno detection and quantification and their immunoaffinity purification. The N-terminal micro sequence has been determined from a print of an IEF gel. By the Polymerase Chain Reaction (PCR) performed on the genomic DNA extracted from the young plant it was possible to select the gene coding for Dac g I (in collaboration with M. Tepfer an F...