Abstract
This chapter focuses on exchange reactions in oxidative phosphorylation. The following exchange reactions are used in studying the mechanism of oxidative phosphorylation: (1) the Pi–ATP exchange, (2) the ADP–ATP exchange, (3) the H2O18–Pi, and (4) H2O18–ATP exchanges. The presence of an exchange reaction does not necessarily prove the existence of the covalent enzyme substrate intermediate, as an exchange reaction could occur between Michaelis complexes. The first measurements of the exchange reaction between ADP and ATP, which can be measured with either C14- or P32-labeled ADP, in connection with oxidative phosphorylation were carried out with preparations from Acetobacter vinelandii. There are two areas in which the exchange reactions have been invaluable: (1) they have been used for the rapid assay of soluble factors that participate in oxidative phosphorylation and (2) the analysis of a partial reaction such as an exchange carries with it the advantages of a simpler system and is particularly useful for the study of the mode of action of uncouplers and inhibitors.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
