Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed β-Propeller Lectin Family.

Lukáš Faltinek,Martina Kašáková,Leonid Kononov,Josef Houser,Nikolay Kondakov,Kamil Parkan,Filip Melicher,Michaela Wimmerová,Sébastien Vidal,Eva Fujdiarová

doi:10.3390/molecules24244540

Abstract

The Photorhabdus species is a Gram-negative bacteria of the family Morganellaceae that is known for its mutualistic relationship with Heterorhabditis nematodes and pathogenicity toward insects. This study is focused on the characterization of the recombinant lectin PLL3 with an origin in P. laumondii subsp. laumondii. PLL3 belongs to the PLL family of lectins with a seven-bladed β-propeller fold. The binding properties of PLL3 were tested by hemagglutination assay, glycan array, isothermal titration calorimetry, and surface plasmon resonance, and its structure was determined by X-ray crystallography. Obtained data revealed that PLL3 binds similar carbohydrates to those that the other PLL family members bind, with some differences in the binding properties. PLL3 exhibited the highest affinity toward l-fucose and its derivatives but was also able to interact with O-methylated glycans and other ligands. Unlike the other members of this family, PLL3 was discovered to be a monomer, which might correspond to a weaker avidity effect compared to homologous lectins. Based on the similarity to the related lectins and their proposed biological function, PLL3 might accompany them during the interaction of P. laumondii with both the nematode partner and the insect host.

Highlights

Photorhabdus is a genus of Gram-negative bacteria belonging to the family Morganellaceae [1].These bacteria are facultative anaerobic rod-shaped microorganisms with bioluminescence ability.Photorhabdus is a subject of many studies due to its interesting life cycle
We introduce a novel l-fucose-binding lectin, which is designated as PLL3
The PLL3 sequence consists of 371 amino acids

Summary

Introduction

Photorhabdus is a genus of Gram-negative bacteria belonging to the family Morganellaceae [1].These bacteria are facultative anaerobic rod-shaped microorganisms with bioluminescence ability.Photorhabdus is a subject of many studies due to its interesting life cycle. Photorhabdus is a genus of Gram-negative bacteria belonging to the family Morganellaceae [1]. These bacteria are facultative anaerobic rod-shaped microorganisms with bioluminescence ability. Photorhabdus is a subject of many studies due to its interesting life cycle. These bacteria have not been observed freely in the soil so far, but a monoculture of certain species of the genus Photorhabdus lives in the gut of nematodes of the genus Heterorhabditis [1,2,3]. Infective juveniles (IJs), a specialized larval stadium of the Heterorhabditis nematode, attack the insect larva and release the Photorhabdus from its intestine into the insect haemocoel.

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