Abstract
BackgroundOvarian cancer is one of the most lethal gynecological malignancies, in which platinum resistance is a common cause of its relapse and death. Glycosylation has been reported to be involved in drug resistance, and glycomic analyses of ovarian cancer may improve our understanding of the mechanisms underlying cancer cell drug resistance and provide potential biomarkers and therapeutic targets.MethodsThe serous ovarian cancer cell line A2780 and its platinum-resistant counterpart A2780-cp were used in this study. We performed a lectin array analysis to compare the glycosylation patterns of the two cell lines, a gene expression array was employed to probe the differences in glycogenes. Furthermore, the results were verified by lectin blots.ResultsA2780-cp cell exhibited stronger intensities of Lens culinaris (LCA) Canavalia ensiformis (ConA), and Lycopersicon esculentum (LEL) and weaker intensities of Sambucus nigra (SNA) lectins. The gene expression array analysis revealed increased expression of Fut8, B3gnt4, B3gnt5, B4galt2 and decreased expression of Fut1 and ST6GalNAc 6 expression were evident in the A2780-cp cells. The lectin blot confirmed the differences in LCA, ConA, SNA and LEL between the A2780 and A2780-cp cells.ConclusionsThe combination of the lectin and gene expression analyses showed that the levels of core fucosylation and poly-LacNAc were increased in the A2780-cp cells and the levels of Fuc α1-2(gal β1-4) GlcNAc and α2-6-linked sialic structures were decreased in the A2780-cp cells. These glycans represent potential biomarkers and might be involved in the mechanism of drug resistance in ovarian cancer.
Highlights
Ovarian cancer is one of the most lethal gynecological malignancies, in which platinum resistance is a common cause of its relapse and death
80% of patients are sensitive to the initial treatment for ovarian cancer, including optimal cytoreductive surgery and subsequent platinum-based chemotherapy, his disease is lethal in 70% of patients because of relapse and drug resistance [5]
We provide an overview of glycan and glycogenes expression in the A2780 and A2780-cp cell lines using a lectin array and a human gene expression array to identify potential biomarkers or targets and provide information on the involvement of glycan in the cisplatin resistance mechanism
Summary
Ovarian cancer is one of the most lethal gynecological malignancies, in which platinum resistance is a common cause of its relapse and death. Ovarian cancer is one of the most lethal gynecological malignancies [1], because of the difficulty of early diagnosis and its recurrence, accompanied by drug resistance [2,3,4]. 80% of patients are sensitive to the initial treatment for ovarian cancer, including optimal cytoreductive surgery and subsequent platinum-based chemotherapy, his disease is lethal in 70% of patients because of relapse and drug resistance [5]. Multidrug resistance phenotype can be reversed by inhibiting the glycosylation of transporter proteins (P-glycoprotein) in ovarian cancer cells [8]. ATP binding cassette transporters in ovarian carcinoma cells that are resistant to oxaliplatin show defective glycosylation and
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
