Abstract
The paper covers study of insufficient water supply effect on lectin activity in different organs of alfalfa plants inoculated with Sinorhizobium meliloti 441 (active strain) and SHM1-48 (inactive strain) during the early stages of symbiosis formation and its active functioning. The correlations between the changes of lectin activity in alfalfa plants influenced by different water supply and strain characteristics, extent of plant development and stage of symbiosis formation.
Highlights
Application of scientific research findings on biological nitrogen fixation into the modern agricultural practices can reduce the cost of energy and raw materials for the production of mineral nitrogen fertilizers and provide an ecologically clean production
The paper covers study of insufficient water supply effect on lectin activity in different organs of alfalfa plants inoculated with Sinorhizobium meliloti 441 and SHM1-48 during the early stages of symbiosis formation and its active functioning
The correlations between the changes of lectin activity in alfalfa plants influenced by different water supply and strain characteristics, extent of plant development and stage of symbiosis formation
Summary
Alfalfa plants of Nadezhda cultivar were grown in sand culture (10 plants / pot) in a 4 kg Wagner containers (washed river sand, moisture content – 60% TMC) at natural lighting, temperature and humidity. Nitrogen fixation activity of root nodules was determined with acetylene reduction method [12]on gas chromatograph “Agilent 6850”. Phytohemagglutins (lectins) were isolated from roots, stems and leaves of alfalfa plants using ethanol elution method [13], with minor modification. Received solution was centrifuged at 6000 rev / min for 15 min. Determination of lectin activity in the received protein solution was performed by hemagglutination reactions in immunological plates with U-shaped holes [14]. Lectin activity was determined after samples incubation with human red blood cells a solution during 2 hours at room temperature corresponding to the biggest dilution, where hemagglutination was observed.
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