Abstract
Miroslav Radman’s far-sighted ideas have penetrated many aspects of our study of the repair of broken eukaryotic chromosomes. For over 35 years my lab has studied different aspects of the repair of chromosomal breaks in the budding yeast, Saccharomyces cerevisiae. From the start, we have made what we thought were novel observations that turned out to have been predicted by Miro’s extraordinary work in the bacterium Escherichia coli and then later in the radiation-resistant Dienococcus radiodurans. In some cases, we have been able to extend some of his ideas a bit further.
Highlights
Miroslav Radman’s far-sighted ideas have penetrated many aspects of our study of the repair of broken eukaryotic chromosomes
These strains lack the deoxyadenine methyltransferase, which modifies DNA at GATC sites and directs mismatch repair to correct the newly copied DNA strand, and lack homologous recombination mediated by the RecA protein
We introduced about 1 mismatched site each 1 kb along the 9-kb region so that we could estimate gene conversion tract lengths associated with crossing-over
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. One of Miro’s stunning discoveries was that dam recA double mutants were extremely sensitive to base-pair substitution mutagens such as 2-amino purine (2-AP) [1] These strains lack the deoxyadenine methyltransferase, which modifies DNA at GATC sites and directs mismatch repair to correct the newly copied DNA strand, and lack homologous recombination mediated by the RecA protein. When the region is flanked by repeated sequences, single-strand annealing (SSA) will result in the deletion of the intervening DNA (D,E) All of these results led us to what we called the “Borts uncertainty principle”: To study the details of crossing-over in meiosis we had to introduce heterologies; but those heterologies altered the outcomes
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