Abstract
A large-scale RNA in vitro selection study systematically identified RNA recognition elements for 205 RNA-binding proteins belonging to families conserved in most eukaryotes.
Highlights
The challenges of characterizing post-transcriptional gene regulation Messenger RNAs are regulated at every stage of their life cycle
Recent proteomic analysis consolidated the number of mRNA-binding proteins (mRBPs) to 700 proteins and revealed at least 20 previously unknown RNA-binding domain (RBD) [1,3]
Various crosslinking and immunoprecipitation (CLIP) methods have been introduced that rely on covalent crosslinking of an RNA-binding protein (RBP) to its RNA targets in live cells, followed by the isolation of crosslinked RBP-RNA segments (Figure 1) [1,6]
Summary
The challenges of characterizing post-transcriptional gene regulation Messenger RNAs (mRNAs) are regulated at every stage of their life cycle. For RBPs with unknown RNA-binding sites, in vitro evolutionary methods (primarily SELEX) that identify high-affinity RNA ligands within pools of randomized sequences have been employed with some success [5].
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