Abstract
In this paper, we describe a novel fabrication method of a microfluidic integrated surface plasmon resonance (SPR) gold chip based on a (3-mercaptopropyl) trimethoxy silane (MPTMS) self-assembled monolayer. This monolayer was formed at the surface of a microfluidic chip made of polydimethylsiloxane (PDMS). Its presence was confirmed by contact angle and Fourier transform infrared spectroscopy measurements on the modified PDMS surface. A basic, but nevertheless appropriate, 4-channel microfluidic system was made on PDMS and reported on a gold SPR sensor. Sealing tests were carried-out by injecting continuous flows of solutions under gradient pressure up to 1.8 bar. Bonding strength of chemical and corona binding were measured and compared. The test of the integrated microfluidic SPR sensor on an SPR bench validated its functionality and proved as well that no leakage is observed between the different microfluidic channels.
Highlights
Highest sensitivity in material permittivity measurements which one of the main adaptations concerns molecular interaction analysis [1,2,3]. When those sensors are converted to biosensors using mainly surface chemistry, the surface plasmon resonance (SPR) detection scheme awards for itself label-free, real-time and extreme kinetics qualifiers that are none the less advantages compared to other molecular interaction detection techniques
PDMS is nicely attaching to glass but the thin 35 nm gold film corresponding to the SPR detection zones appears to be damaged due to the plasma treatment
We present hereby a technique used by Byun et al [13] that is based on silanated thiol-gold bonding process and cumulates the advantages of both physical and chemical techniques, i.e. undamaged and chemically clean SPR channels
Summary
Highest sensitivity in material permittivity measurements which one of the main adaptations concerns molecular interaction analysis [1,2,3] When those sensors are converted to biosensors using mainly surface chemistry, the SPR detection scheme awards for itself label-free, real-time and extreme kinetics qualifiers that are none the less advantages compared to other molecular interaction detection techniques. In the minds building a fully ready-to-use and single-use SPR sensor by embedding the right microfluidic system is still a great challenge It will allow an initial functionalization of SPR sensors before use and greatly simplify the analysis process since only reference and under investigation solutions shall be launched to conduct analyses. PDMS can be considered as the reference material due to its biocompatibility and inertness properties towards biological materials, permeability, flexibility, non-toxicity and easiness in handling It affords optical transparency in the visible spectrum that is always an asset for experiment monitoring. Plasmonic measurements exhibited noisy responses that result from the observable damage of the plasmonic (gold) layer due to the plasma treatment
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