Abstract
The combined antimicrobial effect of glycine and sodium chloride against Escherichia coli IFO3301 was examined in terms of membrane damage. The cellular materials, protein, phospholipid, lipopolysaccharide and 260nm-absorbing materials, leaked from the cells treated with glycine proportionally to the glycine concentration. The leakage was suppressed by the addition of sodium chloride. On the other hand, the largest leakage was observed when the cells grown in 2% sodium chloride-containing medium were treated with glycine. Consequently, the antimicrobial activity of glycine and sodium chloride was not enhanced in the case of combined treatment, but that of glycine was enhanced by the change of the bacterial surface caused by prior sodium chloride treatment during cell growth.The observations by electron microscopy also supported the above results. The membrane of the cells treated with sodium chloride was intact, although a remarkable plasmolysis was observed. On treatment of the cells grown in sodium chloride-containing medium with glycine, extensive blebbing of the membrane and extracellular vesicles was found. The results reflect the injury to the membrane.
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