Abstract
Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC50 of 5.5 µg/mL), ABTS+° scavenging capacity (IC50 of 63.8 µg/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.7 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg/g DW) in the methanolic extract.
Highlights
The genus Phagnalon (Asteraceae) is represented by about 36 species distributed worldwide, six of which are typical of the Mediterranean region [1]
This study aimed to investigate the phenolic profile of P. saxatile leafy stems (LSPSs), which exhibit significant antioxidant activities, through LC-MSn analyses
The extract yields were affected by the solvent used, apolar solvents leading to lower values than methanol and water
Summary
The genus Phagnalon (Asteraceae) is represented by about 36 species distributed worldwide, six of which are typical of the Mediterranean region [1]. Ethnobotanical studies have reported the use of some species of this genus as food ingredient [2] and popular medicine for the treatment of burns [3], renal lithiasis [4], asthma, headache and toothache [5]. Its aerial parts and flowers are traditionally used as an analgesic and to lower blood cholesterol [13] This species has been proposed as a potentially helpful plant to protect against Alzheimer’s disease (AD). The methanolic extract of the flowering aerial parts of P. saxatile were shown to exhibit anticholinesterase activity, cholinesterases constituting a master target for treating AD [9]. The neuroprotective property of P. saxatile extract could be explained by its anti-inflammatory, antioxidant and hypocholesterolemic activities [11]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
