Abstract
In vitro–grown leaves of Malus ×domestica Borkh. cv. Royal Gala were either crush-wounded with forceps, cut, or left whole, and then inoculated with A. tumefaciens strain EHA105 (p35SGUS_INT), with and without vacuum infiltration. Transformation was quantified 13 days after inoculation by determining the rate of β-glucuronidase (GUS) activity. Leaf wounding by crushing with nontraumatic forceps significantly increased transformation when compared with cutting leaves. Vacuum infiltration of inoculum had no effect on transformation.
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