Leaf unfolding and lamina biomechanics in Syngonium podophyllum and Pilea peperomioides

Abstract

In nature, leaves and their laminae vary in shape, appearance and unfolding behaviour. We investigated peltate leaves of two model species with peltate leaves and highly different morphology (Syngonium podophyllum and Pilea peperomioides) and two distinct unfolding patterns via time-lapse recordings: we observed successive unfolding of leaf halves in S. podophyllum and simultaneous unfolding in P. peperomioides. Furthermore, we gathered relevant morphological and biomechanical data in juvenile (unfolding) and adult (fully unfolded) leaves of both species by measuring the thickness and the tensile modulus of both lamina and veins as a measure of their stiffness. In S. podophyllum, lamina and veins stiffen after unfolding, which may facilitate unfolding in the less stiff juvenile lamina. Secondary venation highly contributes to stiffness in the adult lamina of S. podophyllum, while the lamina itself withstands tensile loads best in direction parallel to secondary veins. In contrast, the leaf of P. peperomioides has a higher lamina thickness and small, non-prominent venation and is equally stiff in every region and direction, although, as is the case in S. podophyllum, thickness and stiffness increase during ontogeny of leaves from juvenile to adult. It could be shown that (changes in) lamina thickness and stiffness can be well correlated with the unfolding processes of both model plants, so that we conclude that functional lamina morphology in juvenile and adult leaf stages and the ontogenetic transition while unfolding is highly dependent on biomechanical characteristics, though other factors are also taken into consideration and discussed.