Leaf status and environmental signals jointly regulate proline metabolism in winter oilseed rape.

Marie-Françoise Niogret,Nathalie Marnet,Younes Dellero,Anthoni Pellizzaro,Vanessa Clouet,Alain Bouchereau,Sylvain Dechaumet,Nick Smirnoff

doi:10.1093/jxb/erz538

Abstract

Proline metabolism is an essential component of plant adaptation to multiple environmental stress conditions that is also known to participate in specific developmental phases, particularly in reproductive organs. Recent evidence suggested a possible role for proline catabolism in Brassica napus for nitrogen remobilization processes from source leaves at the vegetative stage. Here, we investigate transcript levels of Δ1-PYRROLINE-5-CARBOXYLATE SYNTHASE (P5CS) and PROLINE DEHYDROGENASE (ProDH) genes at the vegetative stage with respect to net proline biosynthesis and degradation fluxes in leaves having a different sink/source balance. We showed that the underexpression of three P5CS1 genes in source leaves was accompanied by a reduced commitment of de novo assimilated 15N towards proline biosynthesis and an overall depletion of free proline content. We found that the expression of ProDH genes was strongly induced by carbon starvation conditions (dark-induced senescence) compared with early senescing leaves. Our results suggested a role for proline catabolism in B. napus, but acting only at a late stage of senescence. In addition, we also identified some P5CS and ProDH genes that were differentially expressed during multiple processes (leaf status, dark to light transition, and stress response).

Highlights

Winter oilseed rape (Brassica napus L.) is a major oleaginous crop ranked the third most essential source of plant oil in the world
We showed that the underexpression of three P5CS1 genes in source leaves was accompanied by a reduced commitment of de novo assimilated 15N towards proline biosynthesis and an overall depletion of free proline content
All results were expressed on a dry weight basis to compare the different leaf ranks, Decrease of BnaP5CS1 gene expression in source leaves correlates with a decrease of net proline biosynthesis flux

Summary

Introduction

Winter oilseed rape (Brassica napus L.) is a major oleaginous crop ranked the third most essential source of plant oil in the world. Considering the role of ProDH genes during dark-induced senescence in Arabidopsis (Launay et al, 2019), and the specific expression of ProDH2 genes in the midveins of source tissues in B. napus (Faës et al, 2015), proline catabolism might contribute to phloem-dependent N remobilization processes in source leaves of B. napus. Such a role would demonstrate that proline metabolism is a novel target for the improvement of N remobilization efficiency in crops. We identified some BnaP5CS and BnaProDH genes that were differentially expressed during multiple processes (leaf status, dark to light transition, and stress response)

Materials and methods

Results

Discussion