Abstract
Summary A progressive decline of leaf formation capacity was observed in long-term cultures of Arabidopsis callus on 2,4-D. It corresponded to a gradual loss of activity of total peroxidase and of specific isoperoxidases. A passage on media without auxin was required to restore the morphogenetic capacity. It was accompanied by a general increase of activity for most of the isoperoxidases. Leaf formation by passing on a third medium with low IAA level and high kinetin content paralleled a continuous increased enzyme activity as mentioned before by different authors on other materials. The results suggested that morphogenetic potential is dependent upon an endogenous hormonal balance controlled by the exogenous regulators.
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