Leaf development, net assimilation and leaf nitrogen concentrations of five Prunus rootstocks in response to root temperature

Abstract

Rootstocks differentially influence tree physiology and these differences may be due to varying responses to root zone temperature (RZT). To determine if this is the case, the physiology, leaf development and nitrogen relationships of five different Prunus rootstocks with chill requirements between 100 and 1100 h were examined during and after growth at RZTs of 5, 12 and 19 °C for 6 weeks. RZT correlated positively with leaf numbers, expansion rates and final leaf area, and significant differences existed among the rootstocks in the magnitude of these parameters at different RZTs. In particular, leaf expansion and area were less affected at low RZT in the low chill varieties. Net assimilation ( A n), leaf nitrogen ( N%) and photosynthetic nitrogen use efficiency ( A n/ N) also correlated positively with RZT: again, there were differences in the magnitude of these parameters among the rootstocks. No associations amongst A n, N% or A n/ N could be found for the rootstocks; hence, they all differed in their physiological responses to RZT. Low RZT alone was sufficient to reduce A n and decreased both leaf area and photosynthetic activity. Leaf expansion was related to N%, as the varieties with the lowest N% also had the lowest expansion rates. Infrared thermography of the cv. Golden Queen showed a negative correlation between RZT and leaf temperature with leaves of plants at the lowest RZT being 2 °C warmer than ambient whilst those at the highest RZT were 2 °C cooler than ambient. These differences were due to transpiration, as transpiration for the variety used decreased with reducing RZT. Transpiration from the other rootstock varieties was lowest at the 5 °C RZT but, depending on variety, at 12 °C was either higher, lower or the same as that from plants whose roots were at 19 °C. Together, the results of this study explain some of the rootstock-induced changes in tree growth and suggest the need to incorporate seasonal changes in RZT into development models for peaches.