Abstract
Leaves from two Chilean Phaseolus vulgaris landraces cultivated in a growth chamber were assessed for total phenolic (TP), total flavonoid (TF) content and composition, as well as inhibition of α-glucosidase at three different development stages and three sampling times to determine the optimum production of bioactive constituents.The chemical profiles were obtained by HPLC-DAD and UHPLC-MS. We observed qualitative and quantitative differences in the phenolic profile in the Tortola and Negro landraces, with a more complex profile in Tortola. Leaf development and collection time significantly affected (P < 0.05) the phenolic composition and α-glucosidase inhibitory activity. Higher enzyme inhibition was found in mature leaves of the Negro landrace with an IC50 value of 0.55–0.89 μg/mL, regardless of collection time. A significant correlation (P < 0.05) was observed between α-glucosidase inhibition (r = 0.572) and caffeoyl malic acid concentration in the extracts. Our results provide a first approach to the accumulative dynamics of α-glucosidase inhibitors in the leaves of Chilean bean landraces, supporting their possible use as a source of bioactive compounds.
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