Abstract
Investigations were carried out under laboratory conditions in a nutrient solution according to Knop to observe the influence of increasing concentrations of Pb (5, 25, 50, 75 &mu;mol/l) on its uptake and accumulation in chamomile (<I>Matricaria chamomilla</I> L.), diploid cv. Novbona. The essential part of Pb taken up by chamomile plants accumulated in roots; only minor portion of the metal was translocated to the above-ground part of the plant. Addition of Pb to the growth medium reduced significantly the root biomass (–46.3% at the highest supply of Pb); reduction in the above-ground dry matter (–18.3%) was insignificant. Pb treatment also reduced chlorophyll content in leaves (<I>P</I> < 0.01). The highest level of Pb resulted in a decrease of Chl <I>a</I> by 52% and of Chl <I>b</I> by 48%. Lead in the nutrient medium induced accumulation of free proline (Pro) in leaf rosette tissues (<I>P</I> < 0.01). Distribution of Pb in chamomile plants (cv. Novbona) and accumulation of Pb with focus on accumulation in inflorescences (drug <I>Flos chamomillae</I>) was investigated in a pot experiment with soil (Orthic Luvisol) supplemented with 50 mg Pb/kg dry soil. At this treatment, the content of Pb in chamomile inflorescences was 3-fold higher in comparison with the control (<I>P</I> < 0.05) but the level of accumulated Pb (2.08 mg Pb/kg dm flowers) was far below the limit (10 mg/kg) set by the WHO as the highest acceptable level of Pb in the chamomile drug.
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