Lead and cadmium exposure alters shell morphogenesis in a single-chambered, agglutinated foraminiferan protist

Abstract

Changes to calcareous foraminiferal shell morphology are well documented in heavy metal-polluted marine environments, however less is known about how these toxicants affect agglutinated foraminifera, particularly single-chambered (monothalamid) species. Here we used an agglutinated monothalamic foraminifer, Astrammina rara, to study shell morphogenesis during exposure to lead (Pb) and cadmium (Cd). Isolated cell bodies incubated with artificial sediment and control, Pb-, or Cd-spiked artificial seawater constructed new agglutinated shells in four weeks. Time-lapse recordings showed normal motile behavior during treatments. SEM imaging of reconstructed shells, however, revealed dramatic deformations in the bioadhesive of Pb-exposed shells, and less so in Cd-exposed shells. ICP-MS analysis of the isolated shells showed elevated levels of Pb, but not Cd, in the bioadhesive of treated specimens, indicating that the two metals exert their effects differentially. These findings show that certain agglutinated foraminifera may be useful indicator species in studies of heavy metal-polluted benthic marine environments.