結合生物試驗法與LC-MS/MS檢測台灣河川中類雌激素物質之分布

Abstract

Many chemicals in the environment, in particular those with estrogenic activity, can disrupt endocrine signaling pathways. The complex mixture of compounds in the sewage discharged into river may contain xenoestrogenic endocrine-disrupting compounds, which can mimic the action of physiological estrogens on estrogen receptors (ERs) to cause reproductive interference and carcinogenesis in humans. In this study, we detected estrogenic activity in Taiwanese rivers by using a yeast-based reporter gene assay combined with instrumental analysis. Samples were collected from five different rivers of Taiwan, including Danshuei, Yanshuei, Erren, Agnodian, and Gaoping River. Estrogenic activity detected in the Erren River, especially in the tributary (Sanye River), was much higher than other rivers. Xenoestrogenic compounds in the Danshuei River were mainly originated from two tributary rivers (the Dahan and Keelung River), and relatively high estrogenic activity was also found in the midstream and downstream of the Yanshuei River and the Agondian River. Furthermore, estrogenic activity was also detected in the tributary rivers (the Wulo and Niuchou Creek) of the Gaoping River. Xenoestrogenic compounds, including Bisphenol A, Nonylphenol, Estrone, Estriol, and Diethylstilbestrol were found in the Erren River by LC-MS/MS. From this study, bioassay is shown to be a rapid and efficient method to detect xenoestrogenic compounds, but it is necessary to combine with instrumental analysis to identify major xenoestrogenic compounds from environmental samples.