LC-MS/MS determination of ginsenoside compound K and its metabolite 20(S)-protopanaxadiol in human plasma and urine: applications in a clinical study.

Abstract

Ginsenoside compound K (CK) is considered to be a potential therapeutic drug for rheumatoid arthritis because of its good anti-inflammatory activity. The purpose of this work was to establish a rapid, sensitive and specific method for determination of CK and its active metabolite20(S)-protopanaxadiol (20(S)-PPD).Materials& methods:The analytes and internal standards were extracted by liquid-liquid extraction. Then, were separated by high performance liquid phase and determined by triple quadrupole mass spectrometry. A LC-MS/MS using liquid-liquid extraction was developed for determining CK over the concentration range 1.00-1002.00ng/ml and 0.15-54.30ng/ml for 20(S)-PPD. The lower limits of quantification for CK and 20(S)-PPD were 1.00and 0.15ng/ml, respectively. This method was successfully validated for detecting both CK and 20(S)-PPD in the human plasma and urine, and was proved to be suitable for the pharmacokinetic study of CK in healthy Chinese volunteers. ChiCTR-TRC-14004824.