LC-MS/MS method development and validation for the determination of antiviral drug in human plasma

Abstract

The validated protein precipitation method was applied to estimate Darunavir in human plasma with Verapamil as an internal standard. The analytical column was selected Agilent, Zorbax, and XDB C18 (2.1 x 50 mm ID, 5 μm). The column temperature was set at 30°C. The mobile phase composition was acetonitrile:2Mm Ammonium Formate with 0.1% formic acid in water (70:30 v/v). Source flow rate at 0.120 mL/min without a split. An injection volume of 10 µL. Darunavir and Verapamil were eluted at 1.35 &1.13 min, with a total run time of 3.0 min for each sample