Abstract

The aim of the study was to validate a bioanalytical method, for quantifying desloratadine, loratadine and cetirizine in biological samples, useful in toxicological studies. The analytical performance was investigated using the following parameters: selectivity, limit of detection and quantification, linearity, precision, accuracy, matrix effect and analytical stability in the biological matrix. The method optimization was performed for the LC-MS/MS Ultimate 3000 instrument provided by Thermo Fisher Scientific.

Highlights

  • The aim of the study was to validate a bioanalytical method, for quantifying desloratadine, loratadine and cetirizine in biological samples, useful in toxicological studies

  • Hs1t-uadniteihsi,stoanmlyinelosraantaddtihneeir, desloratadine and cetirizine are determined from biological fluids or pharmaceuticals, but there are methods for their simultaneous determination with other compounds found in combinations in certain drugs

  • The size of the interferences was determined for the metabolites of the compounds and for the degradation products that can be formed during the preparation of the sample

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Summary

Introduction

The aim of the study was to validate a bioanalytical method, for quantifying desloratadine, loratadine and cetirizine in biological samples, useful in toxicological studies. The most liquid chromatographic methods for analysis of this compounds reported in the literature are focused on determining metabolites concentrations of [2-11]. Hs1t-uadniteihsi,stoanmlyinelosraantaddtihneeir, desloratadine and cetirizine are determined from biological fluids or pharmaceuticals, but there are methods for their simultaneous determination with other compounds found in combinations in certain drugs (pseudoephedrine). A few methods (capillary electrophoresys, liquid or gas chromatography) have been described in the literature for simultaneously cuantification antihistamines [16-21]. The aim of this study was the development and validation of a simple and sensitive LC-MS/MS method for the simultaneous quantitative determination of three commonly prescribed antihistamines (desloratadine, loratadine, cetirizine) in human plasma. The method can be used clinical cfaosretsh,eaisnvweestlilgaastiofonrotfoHxi1c-aonktiinheistticamainnde related studies concerning the safety of antihistamines

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