Abstract
Oxygenated lipid products of non-cyclooxygenase derivatives, namely, prostanoids such as, isoprostanes and isofurans, are formed in vivo through lipid autoxidation. Insofar it has been marked as novel biomarkers of oxidative stress in the biological systems. Elevations of these oxidized products are associated with several diseases. This chapter describes the preparation and measurement of the products, including newly identified F2-dihomo-isoprostanes and dihomo-isofurans, from plasma and tissue samples using the liquid chromatography-tandem mass spectrometry approach.
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