Abstract
(−)-Epigallocatechin-3-gallate (EGCG) is the main bioactive catechin in green tea. The antitumor activity of EGCG has been confirmed in various types of cancer, including lung cancer. However, the precise underlying mechanisms are still largely unclear. In the present study, we investigated the metabolite changes in A549 cells induced by EGCG in vitro utilizing liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. The result revealed 33 differentially expressed metabolites between untreated and 80 μM EGCG-treated A549 cells. The altered metabolites were involved in the metabolism of glucose, amino acid, nucleotide, glutathione, and vitamin. Two markedly altered pathways, including glycine, serine and threonine metabolism and alanine, aspartate and glutamate metabolism, were identified by MetaboAnalyst 5.0 metabolic pathway analysis. These results may provide potential clues for the intramolecular mechanisms of EGCG’s effect on A549 cells. Our study may contribute to future molecular mechanistic studies of EGCG and the therapeutic application of EGCG in cancer management.
Highlights
As one of the most common cancers globally, lung cancer causes a severe social burden (Ferlay et al, 2015; Siegel et al, 2016)
After exposure to EGCG for 24 h, the A549 cell viability was downregulated in a dose-dependent manner within the liquid chromatographymass spectrometry (LC-MS) Metabolite Profiling of A549 Cells After EGCG Treated
LC-MS of Metabolic Profiles In total, we found that 173 metabolites were reliably detected with relative abundances >1,000 in more than 80% of all samples
Summary
As one of the most common cancers globally, lung cancer causes a severe social burden (Ferlay et al, 2015; Siegel et al, 2016). An estimated 2.2 million new lung cancer cases occurred in 2020 (Sung et al, 2021). As the second most commonly diagnosed cancer, lung cancer remained the leading cause of cancer death, with an estimated 1.8 million deaths in 2020 (Sung et al, 2021). Despite significant advances that have been made in the interventions, including surgery, radiation therapy, chemotherapy, targeted therapy, and immunotherapy on lung cancer, the 5 years survival of lung cancer only remains 21% (Miller et al, 2019; Siegel et al, 2021). Many studies have demonstrated that EGCG may take a role in the initiation, promotion, and progression
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
