Abstract
Magnesium gluconate is widely used pharmaceutical/nutraceutical compound for the prevention and treatment of magnesium deficiency diseases. The present study was designed to explore the effect of The Trivedi Effect<sup>®</sup> - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P<sub>M+1</sub>/P<sub>M</sub> and P<sub>M+2</sub>/P<sub>M</sub>) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect<sup>®</sup> - Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect<sup>®</sup> treated sample. The LC-MS analysis of both the control and Biofield Energy Treated samples indicated the presence of mass of the protonated magnesium gluconate at <i>m/z</i> 415 at the retention time of 1.52 min and fragmentation pattern of both samples were almost identical. The relative peak intensities of the fragment ions were significantly altered in the treated sample compared to the control sample. The proton and carbon signals for CH, CH<sub>2</sub> and CO groups in the proton and carbon NMR spectra of the control and treated samples were found same. The percentage change in the isotopic abundance ratio of P<sub>M+1</sub>/P<sub>M</sub> (<sup>2</sup>H/<sup>1</sup>H or <sup>13</sup>C/<sup>12</sup>C or <sup>17</sup>O/<sup>16</sup>O or <sup>25</sup>Mg/<sup>24</sup>Mg) was significantly decreased in the treated sample by 48.87% compared to the control sample. Subsequently, the isotopic abundance ratio of P<sub>M+2</sub>/P<sub>M</sub> (<sup>18</sup>O/<sup>16</sup>O or <sup>26</sup>Mg/<sup>24</sup>Mg) in the treated sample was significantly increased by 29.18% compared with the control sample. In summary, <sup>13</sup>C, <sup>2</sup>H, <sup>17</sup>O, and <sup>25</sup>Mg contributions from (C<sub>12</sub>H<sub>23</sub>MgO<sub>14</sub>)<sup>+</sup> to <i>m/z</i> 416; <sup>18</sup>O and <sup>26</sup>Mg contributions from (C<sub>12</sub>H<sub>23</sub>MgO<sub>14</sub>)<sup>+</sup> to <i>m/z</i> 417 in the treated sample were significantly altered compared with the control sample. Thus, The Trivedi Effect<sup>®</sup> Treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors using its kinetic isotope effects. Consequently, The Trivedi Effect<sup>®</sup> Treated magnesium gluconate would be valuable for designing better pharmaceutical and/or nutraceutical formulations through its altered physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections.
Highlights
Magnesium gluconate is an organometallic salt of magnesium with gluconic acid for the source of magnesium ion [1]
A sharp and narrow peak was found in both the liquid chromatograms of the control and treated magnesium gluconate (Figure 1) at the retention time (Rt) of 1.52 min indicating that the polarity/affinity of the treated sample remained identical compared to the control sample
The fragment ion peak at m/z 165 corresponding to C5H9O6+ displayed 100% relative peak intensity in the ESIMS spectrum of the control sample, while the highest intense peak was found in the electrospray ionization (ESI)-mass spectrometry (MS) spectrum of the treated sample at m/z 114 corresponding to C5H6O32+ (Figure 2)
Summary
Magnesium gluconate is an organometallic salt of magnesium with gluconic acid for the source of magnesium ion [1]. Magnesium gluconate is used for the prevention and treatment of diabetes mellitus, allergies, cardiovascular diseases, septic shock, inflammatory diseases, immunological disorders, arrhythmias, acute myocardial infarction, gestational hypertension, preeclampsia, eclampsia, Alzheimer's disease, cancer, and oxidative stress induced ischemia/reperfusion injury [4,5,6,7, 9,10,11]. It can be used as neuroprotective [12], an oral tocolytic agent [13], and in a skin-tightening cosmetic composition [14]. Magnesium gluconate is one of the components in this novel proprietary herbomineral formulation as the source of magnesium
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