LC‐DAD‐ESI‐MS/MS characterization of elderberry flower ( Sambucus nigra ) phenolic compounds in ethanol, methanol, and aqueous extracts

Abstract

The aim of the present study is to elucidate the phenolic compositions and antioxidant properties of methanol, ethanol, and aqueous extracts of elderberry flower as well as their sugars, organic acids, and color properties. Phenolic compounds were analyzed with LC-DAD-ESI-MS/MS, while organic acids and sugars were analyzed with LC-DAD-RID. Aqueous extracts were found rich in phenolic compounds and the highest phenolic content was determined in infusions obtained at 100°C for 30 min (922.26 mg/L), whereas the lowest was detected in the ethanol extract for 5 min (238.98 mg/L). Chlorogenic acid, 5-p-coumaroylquinic acid, dicaffeoylquinic acid, and quercetin-3-rutinoside were the most abundant phenolics in all of the extracts.The results revealed significant differences among aqueous, methanol, and ethanol extracts of elderberry flower regarding phenolics, antioxidant, and other chemical properties.Using water infusions at 100°C for 5 and 30 min, respectively, resulted in a higher phenolic and antioxidant potential yield than from using other extractions. Practical Application Elderberry flowers posses a substantial supply of bioactive flavonoids, antioxidant, and phenolic compounds which have frequently been used in traditional medicine and healing. Different solvents and infusion times in extraction might induce differences in phenolic composition and bioactive features. This study highlights that the elderberry flower can be consumed as an alternative herbal tea which is rich in phenolic substances.