LBA01-01 UROPATHOGENS IN THE PROSTATE ARE ASSOCIATED WITH PROSTATE SIZE, INDEPENDENT OF AGE EFFECTS

Abstract

You have accessJournal of UrologyCME1 Apr 2023LBA01-01 UROPATHOGENS IN THE PROSTATE ARE ASSOCIATED WITH PROSTATE SIZE, INDEPENDENT OF AGE EFFECTS Alec Sun, Prajit Khooblall, Juan Sebastian Rodriguez-Alvarez, Smita De, and Aaron Miller Alec SunAlec Sun More articles by this author , Prajit KhooblallPrajit Khooblall More articles by this author , Juan Sebastian Rodriguez-AlvarezJuan Sebastian Rodriguez-Alvarez More articles by this author , Smita DeSmita De More articles by this author , and Aaron MillerAaron Miller More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003360.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Benign prostatic hyperplasia (BPH) affects an estimated 70% of men between the ages of 60-69, yet the etiology is not well understood. Recently, the human urinary tract microbiome has been associated with a variety of urologic diseases, especially those mediated by an inflammatory pathway. Here, we sought to determine associations between age-independent prostate size and microbiome. METHODS: Men over 18 years old undergoing Holmium Laser Enucleation of the Prostate (HoLEP) for BPH with no history of prostate cancer, prostate surgery, or pelvic radiation were recruited. Patients were excluded if they had a positive preprocedural urine culture, recent UTI requiring antibiotics, bladder stones, or if they were catheter-dependent due to obstruction. From each patient, prostate tissue, midstream urine, and urethral and specimen container swabs were collected. All non-prostate samples were used as contamination controls. Patient data such as age, prostate-specific antigen (PSA) level, BPH symptoms, and prostate size were recorded. All samples underwent DNA extraction, 16S sequencing, and analysis in R statistical software. After quality control, reads associated with the controls were removed. High-quality, decontaminated data were assessed for diversity (alpha, beta, taxonomy). The correlation between amplicon sequence variants (ASVs) and patient metrics were quantified through Sparcc correlations, which was designed for count matrix correlations. RESULTS: 20 patients qualified, consented, and were analyzed in this study. Mean age was 68.6 years, mean PSA was 3.4 ng/mL, and mean prostate size was 107.9 g. After bioinformatic decontamination of samples with the negative controls, diversity analyses showed site-specific differences between the urine, urethral swab, and prostate microbiomes were greater than inter-individual variability, indicative of distinct microbiomes. Common uropathogens were positively associated with prostate size. These included five ASVs belonging to Enterobacter cloaceae (p=0.02-0.03), four Planococcaceae ASVs (p=0.008-0.03), and four Acinetobacter (p=0.014-0.026). Only one ASV in the Ralstonia genus exhibited a significant association with age (p=0.015). CONCLUSIONS: This study is the first to characterize the prostatic microbiome in BPH and to link prostate size to specific common bacterial uropathogens while controlling for age and contamination. Further research with a larger sample size and culturomics will provide insight into the mechanisms of how the prostate microbiome contributes to enlarged size. Source of Funding: Endourology Society Summer Student Scholarship © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e1176 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Alec Sun More articles by this author Prajit Khooblall More articles by this author Juan Sebastian Rodriguez-Alvarez More articles by this author Smita De More articles by this author Aaron Miller More articles by this author Expand All Advertisement PDF downloadLoading ...