LB1011 Oncogenic ras mutation induces spatiotemporally specific tissue deformation through converting fluctuated into sustained ERK activation

Abstract

Tissue regeneration and maintenance rely on coordinated stem cell behaviors. This orchestration can be impaired by oncogenic mutations leading to tissue architecture disruption and ultimately cancer formation. However, it is still largely unclear how oncogenes perturb stem cells’ functions to break tissue architecture. Here, we utilized intravital imaging and novel signaling reporter to investigate the mechanisms of oncogenic Kras-induced tissue disruption in the hair follicle. Through longitudinally tracking the same hair follicles in live mice, we found KrasG12D, a mutation that can lead to squamous cell carcinoma, induces epithelial tissue deformation in a spatiotemporally specific manner.