Abstract
The article presents a Layer-by-Layer method to produce new biochip surfaces on gold or mica for protein binding. The chip surfaces were modified by two polyelectrolytes, poly(sodium 4-styrenesulfonate) and pory(diallyldimethylam- monium chloride), which produce negatively and positively charged surfaces, respectively. Then, the modified surfaces were used for the binding of several proteins, such as bovine serum albumine, apo-transferrine, tissue transglutaminase and SAG (sensitive to apoptosis gene) protein. The proteins were self-assembled from aqueous solution on the modified surfaces and adsorption process has been examined by Surface Plasmon Resonance and Atomic Force Microscopy. Our results suggested that negatively charged surface was preferable for the binding of four proteins, even though two surfaces could be used.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
