Layer-by-layer self-assembled films of silk fibroin/collagen/poly (diallyldimethylammonium chloride) as nucleating surface for osseointegration to design coated dental implant materials

Abstract

A coated material with a nucleating surface for osseointegration is an attractive choice for dental implant materials. Layer-by-layer (LbL) self-assembly film coating was used to fabricate a nucleating surface for osseointegration to design coated dental implant materials. Silk fibroin (SF), collagen (Col), and poly (diallyldimethylammonium chloride) (PDDA) were selected as the base materials for the films. LbL self-assembly films of PDDA-SF-PDDA-Col were constructed with different n layers: n = 0 (control), 10, 20, 30, 40, and 50. Quartz crystal microbalance, atomic force microscopy, scanning electron microscopy, and wettability testing were used to analyze the multilayer formation, topography, morphology, and surface characteristics of the films, respectively. Molecular organization of the films was characterized by Fourier transform infrared and Raman spectroscopy. The biological performance was evaluated with osteoblast cell proliferation, alkaline phosphatase (ALP) activity, and total protein absorption. The multilayer showed a linear function of thickness and the number of layers. The multilayer films demonstrated mobility of amide I, II, III, and molecular skeletal vibration. The films had rough surfaces, hydrophilicity, biological performance to enhance cell proliferation, ALP activity, and total protein absorption. The results indicated the films were promising as a nucleating surface for osseointegration in the design of coated dental implant materials.