Abstract
Gene vectors provide a viable approach to ensuring that therapeutic genes reach the target cells. Polyethyleneimine (PEI), a cationic polymer, was brought into focus on high transfection efficiency as a gene carrier. However, the high cytotoxicity and lack of targeting ability during loading genes remain confusing topics. Herein, Fe3O4 served as the magnetic source, plant extract Zein served as the structural unit loaded with PEI and hyaluronic acid (HA) served as the targeted switch, the Fe3O4/Zein/PEI25k-HA (FeZP25k-HA) was synthesized through an antisolvent approach, and its cytotoxicity, targeting ability and transfection efficiency in HEK 293 T and HeLa cells were further investigated. The FeZP25k-HA exhibited low toxicity at the cellular level, and under external magnetic fields, it revealed a significant enrichment effect, which contributed to enhancing cellular uptake. The FeZP25k (Fe3O4/Zein/PEI25k) modified with HA displayed higher uptake and transfection efficiency in HeLa cells, and excellent tumor targeting ability. This work was expected to offer an innovative strategy to design a safe, efficient magnetic targeted gene delivery system with low toxicity and high transfection efficiency.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
