Layer-by-layer films of polysaccharides modified with poly(N-vinylpyrrolidone) and poly(vinyl alcohol)

Kanstantsin S Livanovich,Anastasiya A Sharamet,Anna N Shimko,Tatsiana G Shutava

doi:10.1016/j.heliyon.2021.e08224

Kanstantsin S Livanovich, Anastasiya A Sharamet + Show 2 more

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https://doi.org/10.1016/j.heliyon.2021.e08224

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Journal: Heliyon	Publication Date: Oct 1, 2021
Citations: 1	License type: cc-by

Affiliation: National Academy of Sciences of Belarus

Abstract

N-grafted copolymers of chitosan (460 kDa) with poly(N-vinylpyrrolidone) (2.4 kDa) or poly(vinyl alcohol) (2.0 kDa) as side chains were synthesized. Depending on the polymer-to-chitosan mass ratio the degree of amino group substitution with side chains in chitosan backbone was varied in the range of 0.01–0.33. Layer-by-layer films consisted of copolymers and dextran sulfate as polyanion were obtained. Thickness, hydrophilicity, and morphology of the films were investigated using QCM, UV-vis spectrophotometry, AFM, and contact angle measurements. The obtained films show enhanced protein-repellent properties in fetal bovine serum medium. The mass of adsorbed proteins on LbL films based on copolymer with a degree of substitution of 0.2 decreases by 50 % compared to unmodified chitosan. Protein-repellent properties of copolymer-based films are common for LbL films of grafted chitosan copolymers and depend on hydrophilic side chain density on the surface.

Highlights

Formation of protein-resistant coating is an essential topic in biomimetic surface engineering
In this work we report on the preparation of LbL films based on graft copolymers of chitosan with PVP or poly(vinyl alcohol) (PVA) and dextran sulfate and characterization of their thickness, hydrophilicity, surface roughness, and protein repellency (Figure 1)
Chitosan is covalently modified with side chains of PVP 2.4 kDa or PVA 2.0 kDa and graft-copolymers CH-SUB with various degree of amine group substitution by the short polyvinyl side chains are obtained

Summary

Introduction

Formation of protein-resistant coating is an essential topic in biomimetic surface engineering. Nonspecific protein adsorption on artificial surfaces of catheters, implants, nanoparticles, etc. To avoid the immune response the surfaces are modified through plasma etching, chemical vapor deposition or phy--sisorption of biomimetic species (proteins, cells, hydrogels, polymers) [1, 2, 3]. One of the most effective methods to reduce the immune response on artificial implants is the chemisorption of polyethylene glycol (PEG) chains to form the hydrophilic brushes preventing nonspecific adsorption [1, 4]. The addition of PVP 10 kDa to ammonium hydroxide solution prior the sol-gel synthesis produces stable polymer-capped SiO2 nanoparticles for adsorption of bilirubin [7]. Carboxylated by NaOH treatment PVP was used in preparation of stable silver nanoparticles (AgNP) conjugated with cytokine that show high anti-inflammatory effectiveness in vitro [9]. The capping with PVP remarkably improves the stability of hybrid AgNP in protein-containing environments especially in bovine serum albumin (BSA) solutions [11]

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