Layer-by-Layer Deposition of Hyaluronan and Quercetin-Loaded Chitosan Nanoparticles onto Titanium for Improving Blood Compatibility

Xingda Wu,Nan Tang,Yanfang Zhang,Cuijuan Liu,Lin Li,Hongpeng Chen

doi:10.3390/coatings10030256

Xingda Wu, Nan Tang + Show 4 more

Open Access

https://doi.org/10.3390/coatings10030256

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Journal: Coatings	Publication Date: Mar 9, 2020
Citations: 12	License type: CC BY 4.0

Affiliation: Guangdong Medical College, Southern Medical University

Abstract

Surface modification is an effective way to improve the hemocompatibility of biomaterials. Quercetin has significant anticoagulation and antithrombotic effects, and thus it is a promising candidate agent for the surface modification of blood-contacting materials. In this study, quercetin was successfully encapsulated in tripolyphosphate–chitosan nanoparticles (TCs) based on the ionic gelation of chitosan with tripolyphosphate (TPP) anions. Then, hyaluronan acid (HA)/quercetin-loaded TPP–chitosan nanoparticle (QTCs) films, in addition to HA/TCs films, were prepared separately using an electrostatic layer-by-layer self-assembly technique. The encapsulation of quercetin in the chitosan nanoparticles was confirmed by UV spectra. The quercetin-loaded multilayer coatings were also successfully self-assembled, as confirmed by the UV spectra and contact angle measurements. Platelet adhesion experiments were carried out with platelet-enriched plasma so as to evaluate the blood compatibility of the different samples. There were many platelets on the surfaces of the glass and HA/TC-coated titanium, which were partially activated but not aggregated. Meanwhile, many more platelets were observed on the uncoated titanium surfaces, most of which developed pseudopodia. By contrast, the platelet adhesion and activation were reduced remarkably on the surface of the HA/QTC-coated titanium. These results showed that the multilayer coatings containing quercetin could act as potential biomaterials to improve the anticoagulation performance of blood-contacting materials.

Highlights

In the design of blood-contacting implants, such as artificial blood vessels and vascular stents, there are two major issues that should be considered, namely, antithrombosis and endothelialization [1].Native endothelium plays a unique role in maintaining vascular homeostasis, which includes active antithrombosis, inhibiting the proliferation of smooth muscle cells, and reducing intimal hyperplasia [2,3]
After the incorporation of the the liquid switched to a translucent state with a weak opalescence, and the yellowish color seemed to TPP solution, the liquid switched to a translucent state with a weak opalescence, and the yellowish disappear, as shown in
Quercetin-loaded chitosan nanoparticles were fabricated based on the ionic interactions between chitosan and TPP anions

Summary

Introduction

In the design of blood-contacting implants, such as artificial blood vessels and vascular stents, there are two major issues that should be considered, namely, antithrombosis and endothelialization [1]. Native endothelium plays a unique role in maintaining vascular homeostasis, which includes active antithrombosis, inhibiting the proliferation of smooth muscle cells, and reducing intimal hyperplasia [2,3]. The endothelialization of implant surfaces may provide fully blood-compatible interfaces. We know that it takes some time to complete the endothelialization of implant surfaces in vivo. The blood compatibility of an implant is the first consideration, especially in the initial implantation.

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Conclusion