Abstract
Background and Objectives: This study aimed at investigating the laxative effects of a standardized aqueous extract of Dendropanax morbiferus H. Lév. on two different constipation rat models. Materials and Methods: Animal studies were conducted with low-fiber diet-induced and loperamide-induced constipation animal models, and isolated colons were used in ex vivo analysis to determine the changes in colonic motility caused by D. morbiferus H. Lév. leaf extract (DPL). Results: The results showed that DPL administration significantly improved certain reduced fecal parameters (number, weight, and water content of the stools) in a both low-fiber diet and loperamide-induced constipation models without adverse effects of diarrhea. The laxative effect of DPL was confirmed to improve the charcoal excretion time upon DPL treatment in a low-fiber diet or loperamide-induced constipation model through gastrointestinal (GI) motility evaluation using the charcoal meal test. In addition, when DPL was administered to RAW264.7 cells and loperamide-induced constipation model rats, the production of prostaglandin E2 (PGE2) increased significantly in cells and tissue. Furthermore, DPL dose-dependently stimulated the spontaneous contractile amplitude and frequency of the isolated rat colon. Conclusion: Although our study did not provide information on the acute or chronic toxicity of DPL, our results demonstrated that DPL can effectively promote defecation frequency and rat colon contraction, providing scientific evidence to support the use of DPL as a therapeutic application. However, further toxicity studies of DPL are needed prior to the initiation of clinical trials and clinical applications.
Highlights
Constipation is defined as a gastrointestinal disorder characterized by difficult, irregular, or deficient defecation [1]
A similar pattern was observed in the therapeutic test (Table 4) on GI motility. These results demonstrate that DPL treatment can enhance GI motility in the loperamide-induced constipation rat model
Our results showed significant increases in fecal number, weight, and water content following the administration of bisacodyl in rat models of low-fiber diet-induced (Figure 5) and loperamide-induced (Figure 7) constipation
Summary
Constipation is defined as a gastrointestinal disorder characterized by difficult, irregular, or deficient defecation [1]. The aim of the present study was to confirm the preventive and therapeutic effects of DPL on low-fiber diet- and loperamide-induced constipation rat models in vivo and the modulatory effects of DPL on the contraction of ex vivo colonic smooth muscle in rats. This study aimed at investigating the laxative effects of a standardized aqueous extract of Dendropanax morbiferus H. Materials and Methods: Animal studies were conducted with low-fiber diet-induced and loperamide-induced constipation animal models, and isolated colons were used in ex vivo analysis to determine the changes in colonic motility caused by D. morbiferus H. Results: The results showed that DPL administration significantly improved certain reduced fecal parameters (number, weight, and water content of the stools) in a both low-fiber diet and loperamide-induced constipation models without adverse effects of diarrhea. Further toxicity studies of DPL are needed prior to the initiation of clinical trials and clinical applications
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
