Latex agglutination test for detection of mutans streptococci in relation to dental caries in children

Abstract

A simple and rapid system based on a latex agglutination (LA) reaction was devised for the detection of mutans streptococci in dental plaque. Latex particles were sensitized with antibodies against whole cells of Streptococcus mutans strains MT8148 (serotype c), MT703R ( e) and OMZ175 ( f) and Strep. sobrinus strains B13 ( d) and 6715 ( g). These sensitized particles were agglutinated within a few minutes after addition of 1.0–10ng serotype-specific antigen from the homologous organisms or the nitrous acid extract of whole cells at 10 5–10 6c.f.u. The LA test specifically differentiated not only mutans streptococci from the other oral streptococci but also Strep. sobrinus from Strep. mutans. The LA test was also applicable to extracts of plaque from 206 human subjects who harboured mutans streptococci. In clinical trials, the outcome of the LA test correlated significantly with the number of mutans streptococci found in plaque ( p < 0.0001), which was quantified by the selective cultivation of mutans streptococci. Furthermore, the LA test discriminated between Strep. mutans and Strep. sobrinus from human dental plaque. The sensitivity and the specificity of the LA test for detection of mutans streptococci were 78.9 and 100%. The degree of reactivity in the LA test correlated significantly with the number of decayed tooth surfaces ( p < 0.0001) and decayed and filled tooth surfaces ( p < 0.0001). These results suggest that the LA test could be useful clinically for the detection of mutans streptococci in dental plaque as well as serving as a caries-activity test.