Laterality in cavefish: Left or right foraging behavior in Astyanax mexicanus

Abstract

The evolution of foraging behaviors is key to colonizing challenging habitats such as a cave’s dark environment. Vibration attraction behavior (VAB) gives fish the ability to swim in the darkness toward a vibration stimulus produced by many prey crustaceans and insects. VAB evolved in the blind Mexican cave tetra, Astyanax mexicanus. VAB is regulated by an increased number of mechanosensory neuromasts, particularly in the eye orbital region. However, VAB in Astyanax is only correlated with the number of neuromasts on the left side. Astyanax also have a bent skull preferentially to the left and a QTL signal for the right-side number of neuromasts. We conducted field studies in five different cave populations for four years. Results support that all cave populations can express behavioral lateralization or preponderance of side to examine a vibrating object. The percentage of individuals favoring one side may change among pools and years. In one cave population (Pachón), for one year, this “handedness” was expressed by preferentially using the right side of their face. On the contrary, in most years and pools, Tinaja, Sabinos, Molino, and Toro cave populations explored preferentially using their left side. This suggests that if there is an adaptative effect, it selects for asymmetry on itself, and not necessarily for which side is the one to be specialized. Results also showed that the laterality varied depending on how responsive an individual fish was, perhaps due to its nutritional, motivational state, or mode of stimuli most relevant at the time for the fish.