Abstract
A lateral flow immunoassay for sensitive detection of skeletal troponin I (TnI) as a specific, thermostable marker of muscle tissue was developed. Due to the antibodies’ choice, the assay specifically detects mammalian TnI (in beef, pork, lamb, and horse) but does not detect bird TnI (in chicken or turkey), thus enabling differentiation of these types of raw meat materials. The assay is based on a sandwich format of the analysis using gold nanoparticles as labels. The time of the assay is 15 min, and the TnI detection limit is 25 ng/mL. A buffer solution is proposed for efficient extraction of TnI from muscle tissues and from finished meat products that have undergone technological processing (smoking–cooking–smoking, cooking and smoking). The possibility of detecting beef addition in minced chicken down to 1% was demonstrated.
Highlights
In recent decades, the rapid control of the composition of food products and identification of undeclared components has been an acute issue
Bovine skeletal troponin I and monoclonal antibodies against TnI clones IS7 and 6F9 were purchased from HyTest (Turku, Finland, hytest.fi)
IgG
Summary
The rapid control of the composition of food products and identification of undeclared components has been an acute issue. Consumers worldwide are concerned about mass falsifications of food products, in particular meat products, as a result of numerous reports highlighting potential risks to human health and serious financial losses [1,2]. Falsifications of meat foodstuffs change the properties of finished products, and can violate consumer preferences or dietary restrictions and be dangerous to people’s health and well-being (e.g., halal products, allergenic compounds). Rapid and productive analytical methods are necessary to control the composition of meat products. Microstructural or histological methods are not effective for the analysis of finished meat products that have gone through numerous stages of technological processes. Electrophoresis, and various types of chromatography cannot be used as screening methods because they are laborious and time-intensive and their implementation requires special laboratories, sophisticated equipment and highly skilled personnel [4]
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