Abstract
Simple, inexpensive, and high sensitive Lateral Flow Immunoassay (LFI) based colloidal gold nanoparticles antigen-coated as the label was designed for the fast determination of organophosphorus pesticide in cow’s milk samples. The developed LFI detection strip consisted of four structural zones start from the sample pad, conjugate pad, detection zone, and finally absorbent pad. Immune-sensitive zone was synthesized and functionalized with Propanoic Hapten–OVA, Goat monoclonal-anti-Propanoic antibodies were produced. Propionic -cationized ovalbumin-HPR conjugates were fabricated and identified. The antibody optimal conditions were set and the influence of pH on the analytical properties of the antibody-based gold nanoparticles was also investigated. The detection limit of the LFI for Propanoic standard into milk samples were 0.5 and 100 ng/mL, respectively. The test development time for the LFI was less than 5 min, suitable for fast on-site measuring of Propanoic.
Highlights
The lateral flow immunoassay (LFI) is a paper-based technique for the determination and measurement of particular substances within a complex mixture, by putting the sample at a test tool and the outcomes will be displayed within 5–30 minutes [1]
Pesticides need high valid and accurate methods to detect their levels in food, soil, and water like GC or HPLC with mass spectrometry that are high-cost equipment, required a well-trained technician, a significant time for sample preparation, weakness to operate under field conditions [6]
The immune test based on the adhesive of an antigen particle to distinct antibodies, the conducted test may be only using one antibody or multiple using by many antibodies [10, 11]
Summary
The lateral flow immunoassay (LFI) is a paper-based technique for the determination and measurement of particular substances within a complex mixture, by putting the sample at a test tool and the outcomes will be displayed within 5–30 minutes [1]. This method is distinguished by low costs, ease of production, rapid and portable, and popular applications in biomedicine, agriculture, food, and environmental sciences [2]. A day the interest has been growing in diagnostic with various test lines allowing the quick self-examination of multiple analytes present in samples [3] Such assays (potentially a private LFIA) should be simple to work without the use of laboratory examination or individuals qualified in chemical analyses [4]. The main objective of this study is to develop fast and sensitive immunosorbent assay based on gold nanoparticles using monoclonal antibody for determination of organophosphorus pesticide (Propanoic)
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