Abstract
We have developed a self-assembled fluorescent cluster comprising a seminaphthorhodafluor (SNARF) derivative protected by a photoremovable o-nitrobenzyl group. Prior to UV irradiation, a colorless and nonfluorescent cluster was spontaneously assembled in aqueous solution. After UV irradiation, the self-assembled cluster remained intact and showed a large enhancement in pH-responsive fluorescence. The unique pH responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe not only in the test tube but also in HeLa cell cultures.
Highlights
Fluorescence imaging is one of the most powerful techniques for the real-time, noninvasive monitoring of biomolecules and cellular processes in living systems with high spatial and temporal resolution, through direct monitoring of the behaviors of fluorescent molecules
Based on the foregoing discussion, we recently reported a rational strategy for the design of a latent ratiometric fluorescent probe for monitoring intracellular pH, i.e. a latent ratiometric fluorescent pHi probe,[10,11,12,13] since tracking the dynamics of pHi is crucial to understanding the regulation mechanisms of several physiological functions of cells and tissues. [14,15] It is well known that seminaphthorhodafluor
These results indicated that the fluorescent cluster has unique characteristics as a dual-emissive ratiometric fluorescent pH probe (Figure 2(d))
Summary
Fluorescence imaging is one of the most powerful techniques for the real-time, noninvasive monitoring of biomolecules and cellular processes in living systems with high spatial and temporal resolution, through direct monitoring of the behaviors of fluorescent molecules. In contrast to our previous knowledge, the UV-irradiated uncaged-SNARF derivatives maintained the self-assembled cluster state, but the cluster showed strong pH-responsive fluorescence. This unique pH-responsive fluorescent cluster could be used as a dual-emissive ratiometric fluorescent pH probe in both test tube and cell cultures.
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