Abstract
Materials and methods The rTg4510 mice express the TauP301L mutation under control of a tetracycline-responsive operon promoter. rTg4510, single transgenic tTA and non-transgenic littermates (129S6/FVB/N F1 hybrid) were bred at Taconic, Denmark. Paraffin sections were stained for p-Tau, astroand microglial markers, ubiquitin, doublecortin and tangles. Western blot was performed on soluble (S1) and sarkosyl-insoluble (P3) brain fractions. Hippocampal CA1 neuron numbers and cortical neuronal densities were assessed by un-biased stereology at Gubra, Denmark.
Highlights
The rTg4510 mouse overexpresses an inducible human mutant tau (TauP301L) selectively in the forebrain
We detected a significant decrease in CA1 neuron number compared to both non-transgenic and tTA mice from 10W
To further investigate a potential developmental effect in our transgenic mice, brain sections from all 3 genotypes were stained for doublecortin
Summary
Late neurodegeneration in the Tau transgenic mouse model rTg4510 Background The rTg4510 mouse overexpresses an inducible human mutant tau (TauP301L) selectively in the forebrain. This mouse exhibits Tau hyperphosphorylation at AD-relevant epitopes, accumulation of the 64 kDa tau species, and neurofibrillary tangles in cortex and hippocampus. Paraffin sections were stained for p-Tau, astroand microglial markers, ubiquitin, doublecortin and tangles.
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