Abstract
To evaluate macro and microscopically the late evolution of autotransplants of fragments of spleen in the greater omentum, mesenterium and peritoneal cavity, after 24 weeks of observation. Fifty two Wistar rats were used, males and adults, submitted to total splenectomy and divided in four groups. The group I--seventeen animals with implant of spleen fragment in the peritoneal cavity; group II--eighteen animals with implant in the omentum and group III--seventeen animals with implant fixed in mesenterium root. The group control (group IV) was formed by eight animals chosen aleatorily among the three groups. It was analyzed macro and microscopically the evolution of the implant, and in the histological study qualitative and quantitative criteria were adopted, with the counting of no cellular e cellular elements. It was observed adherences to the adjacent tissues and vascularization in all of the fragments transplanted. The group I presented white pulp and preserved vascularization. In the group II were observed white pulp with follicular formations and lymphoid tissue preserved, and the red pulp in cordon aspect and hemorrhagic. In the group III were observed with depletion of white and red pulp, while others evidenced better preservation of the pulps. The counting of lymphocytes revealed significant difference between the groups I and IV and the group III and IV (p < 0.05). The counting of active macrophages revealed significant difference between the groups II and III (p < 0.05) and similarity between II and IV (p > 0.05). The other elements: active macrophages phagocyting hemosiderine, plasmocytes, fibroblasts, fibrocytes, giant cells, monocytes, interstitial spaces and fibers of collagen, did not show significant difference among the groups. The splenic autotransplantation is feasible, being the better place the greater omentum. This research demonstrated through qualitative and quantitative histological analysis that the splenic tissue autotransplanted in the omentum of Wistar rats preserves its function of defense of the organisms.
Highlights
The spleen is a lymphoid organ that plays important part in the organism defense, participating in filtration processes, phagocytosis and immunoglobulin production
The animals divided in three groups were submitted to total splenectomy and intra-abdominal spleen tissue autotransplantation, being the Group I with seventeen animals and implant in the cavity; Group II with eighteen animals and implant in the omentum and Group III with seventeen animals and implant in the mesenterium root
Important reduction was registered in the weight of the splenic tissue implanted in the three groups, being of 31%, 34.6% and 50.3%, respectively in the groups I, II and III
Summary
The spleen is a lymphoid organ that plays important part in the organism defense, participating in filtration processes, phagocytosis and immunoglobulin production. Their main functions are: haematopoietic, immunological in the production of lymphocytes, plasmocytes and macrophages, phagocytosis denominated of haemocatheresis and it is part of the “pool’’ outlying of blood storage[1,2]. The spleen is formed by a stroma and the parenquima being its cellular arrangement differentiated in white and red pulps. The main cellular types in the splenic structure are: T and B lymphocytes, macrophages, plasmocytes, fibrocytes, reticulocytes and dendritic cells[1,2]
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