Abstract

The LIM and SH3 protein 1 (LASP-1) has been reported to be associated with tumor development and progression. However, the expression and potential function of LASP-1 in human non-small cell lung cancer (NSCLC) remains undefined. Thus, this study aims to determine the relationship of LASP-1 expression with the progression and prognosis of NSCLC. Expression of LASP-1 was evaluated in NSCLC tissues and cell lines by real-time PCR, immunohistochemistry and Western blot analysis. The relationship between LASP-1 expression and clinicopathological characteristics was analyzed. The effects of LASP-1 on cell proliferation, migration and invasion were investigated in NSCLC cell lines in vitro and in vivo. Luciferase assay was used to determine whether LASP-1 could be regulated by miR-203. We found that LASP-1 was overexpressed in NSCLC and its expression level was closely correlated with tumor size, advanced TNM stage, lymph node metastasis as well as survival time and could be recognized as an independent prognostic factor of patients. LASP-1 could promote proliferation, migration and invasion of NSCLC cells in vitro and in vivo. Moreover, LASP-1 was proved to be a direct target gene for miR-203. Our results suggest that LASP-1, mediated by miR-203, has crucial functions in the proliferation, migration and invasion of NSCLC.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.