Lasers for matrix‐assisted laser desorption ionization

Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was introduced over 30 years ago, and it now plays a key role in several areas, including the characterization of microorganisms and tissue imaging. Lasers are, of course, a central component in MALDI mass spectrometers, but in commercial systems, the type of laser is determined by the instrument manufacturer, and for the operator, their only practical consideration is the selection of the optimal laser power, or fluence, that gives them the best quality spectrum for a given sample and matrix combination. In the Special Feature this month, Dr. Kermit K. Murray, Department of Chemistry, Louisiana State University, Baton Rouge, LA, provides a detailed discussion of the laser and its role in matrix-assisted laser desorption/ionization. Dr Murray's article includes a brief history of the development and use of lasers for ionization in mass spectrometry and he describes the wide range of lasers, wavelengths and instrument configurations, including dual-pulse configurations, that have been developed and evaluated during the evolution of MALDI mass spectrometry. For any mass spectrometrist using MALDI MS in their work, this Special Feature is essential background reading on one of the most important, but poorly understood components of this powerful analytical tool.