Laser trapping of Ag nanoparticles to enhance Raman spectroscopy in aqueous media.

Abstract

Laser trapping (LT) of metallic nanoparticles (NPs) is an approach that has the potential to enhance Raman spectroscopy in aqueous media. In this paper, we report the LT of multiple 60-nm Ag NPs using a tightly focused 1064-nm Gaussian laser beam. The dynamic process (trapping and escaping) of the individual Ag NPs were recorded using a charge coupled device (CCD) camera in backscattering illumination mode. We found that up to four Ag NPs could be simultaneously trapped; however, they were unstable in the laser trap due to Brownian motion and NP-NP interactions. However, after mixing Ag NPs with Bacillus subtilis, more of the Ag NPs could be trapped together with the bacteria. Furthermore, a 532-nm solid-state laser beam was used to activate Raman scattering of the Ag NPs + Bacillus subtilis sample. Based on repetitive measurements, the Raman spectra of the Ag NPs + Bacillus subtilis sample were enhanced and the results were consistent. Our work suggests that LT of metallic NPs can be used to enhance Raman spectroscopy in aqueous media. We believe that the enhanced Raman spectroscopy will be useful for real-time biological assays.